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Cryo Techniques

Freeze Fracture

The JFD-9010 freeze etching equipment is used to prepare specimens for electron microscopy by various treatments of fresh biological specimens frozen by rapid freezing. The treatments include freeze fracturing and freeze-etching in high vacuum for maintaining cells and tissues of such specimens in an almost native state, plus electron beam evaporation for making replica films of ultra-micro particles.

Also, use of electron beam evaporator and specimen rotation/tilt mechanism allows low-angle rotational evaporation to be performed with ease. This is extremely useful for molecular biologists who employ electron microscope for high-resolution observation of protein molecules.

Cryo ultramicotomy

1. Sectioning down to -185 ° C.

2. New contact-free through-the-wall specimen holder system for chatter free cryosection.

3. Open top design with no disturbing ice-condensation inside or outside during or after work.

4. Attaches to the Ultracut S and is ready for sectioning at low temperature within a few minutes.

5. Memory for 3x3 settings of knife specimen and gas temperature.

6. Continuous turbulence-free no-pressure LN2 filling system with a very low LN2 consumption.

7. New shell mounted sectioning chamber with armrest for comfortable manipulation of sections.

8. Accurate control of specimen, knife and the most important chamber gas temperature.

9. Backlight illumination for accurate glass and safe diamond knife approach.

10. Alignment of knife with self-locking drives from outside the chamber.

Cryo system in FESEM

-Cryo SEM with Alto 2500 has been specifically designed to allow highest resolution performance together with Field Emission SEMs for  imaging and analysis of biological and other hydrated, beam or vacuum sensitive specimens.
The cryo-method has the great advantage that the material is so rapidly frozen that vulnerable biological structures are well preserved and that when using Cryo-fracture the material breaks clean along weak edges (e.g. membranes) without causing much malformation.

-The Bright Starlet is the world's smallest cryostat! It is a small, portable and convenient instrument with a choice of handwheel or lever operation. The compact chamber ensures rapid cool-down times, is easy-to-clean and resists common decontaminating agents. An insulated cover is used to ensure maximum efficiency of the refrigeration system and to reduce frosting when the unit is not in use. If the unit is transported, the chamber will remain cold for a prolonged period.
 

 

Starlet 2212 Cryostat Features:

1. Single refrigeration system (to -30 °C)

2. Manual microtome with 2-12µm feed

3. Magnacut blade holder with long-life disposable blades

4. Magnaplate anti-roll plate system

5. Handwheel or lever operation
 

6. Specimen quick freeze (-40 °C) with digital display

7. Programmable automatic defrost

An Ultraviolet Light Decontamination Unit option is available for the Bright Starlet 2212 Cryostat